Strain selection during serial passage of Trichoplusia in nuclear polyhedrosis virus
نویسندگان
چکیده
منابع مشابه
Characterization of DNA Polymerase Activity in Trichoplusia ni Cells Infected with Autographa californica Nuclear Polyhedrosis Virus
Nuclei isolated from Trichoplusia ni cells (TN-368) infected with Autographa californica nuclear polyhedrosis virus (AcMNPV) were used to study the replication of viral DNA. Based on DNA : DNA hybridization data, virus-specific DNA polymerase activity was insensitive to aphidicolin and novobiocin and was inhibited by ddTTP and N-ethylmaleimide. The data indicate that the AcMNPV-specific DNA pol...
متن کاملReassortment of simian virus 40 DNA during serial undiluted passage.
Alterations occur in the supercoiled form of viral DNA after the serial undiluted passaging of simian virus (SV) 40. We have identified a portion of the viral genome which is amplified during this process. These SV40 DNA sequences represent about 30% of the viral genetic information and are present in a reiterated form in twisted circular molecules prepared from purified virions. In addition, r...
متن کاملBaculovirus Replication: Stimulation of Thymidine Kinase and DNA Polymerase Activities in Spodopterafrugiperda Cells Infected with Trichoplusia ni Nuclear Polyhedrosis Virus
Baculoviruses are large DNA-containing viruses which replicate in the nucleus of invertebrate cells. The viruses contain supercoiled DNA of tool. wt. about 8 x l 0 7 (Bud & Kelly, 1977) and the multiply enveloped nuclear polyhedrosis virus of Trichoplusia ni used in this study has a genome size of 8.3 x 107 (Bud & Kelly, 1980). Little is known about the replication of baculoviruses in molecular...
متن کاملCharacterization of nuclear polyhedrosis virus DNAs.
The nuclear polyhedrosis virus DNAs characterized and compared in this study consist of the singly-enveloped nucleocapsids (SNPV) of Trichoplusia ni and the bundles of nucleocapsids common to a single envelope (MNPV) from Spodoptera frugiperda and Rachiplusia ou. The SNPV and MNPV DNAs are very similar in hydrodynamic properties and molecular weights. In addition, the NPV DNAs are similar in si...
متن کاملVirus envelope acquisition of nuclear polyhedrosis virus in vitro.
There have been several reports concerned with the replication and morphogenesis of insect baculoviruses during the past decade [1--7]. While there is general agreement as to the assembly of the virus on the basis of electron microscopic studies, there are still questions regarding the details of the replicative mechanisms, such as the acquisition of the virus envelope. Three possible ways have...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1976
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.18.3.1040-1050.1976